animal cell culture

Mammalian Cell Culture Technology

Mammalian cell cultures are cultured in industrial bioreactors to manufacture viral vaccines, immunobiologicals, hormones, therapeutic enzymes, etc. The high growth in this sector has created a gap for skilled cell culture process technologists. To create interest among students to pursue this exciting field of animal cell culture we have created course  focusing on aseptic handling techniques ,its application in industry and recent technological advances.

Basic Program: 1 Week Duration

Course Code: MM 01

Practical Learning Objectives:

  • Learn aseptic techniques involved in handling of animal cell cultures
  • Gain practical skills to subculture  animal cells and quantify cell growth
  • Apply sterile filtration for media preparation and optimize cell growth parameters
  • Understand principles of cryopreservation
  • Learn cell banking methods employed in industry
  • Comprehend principles of bioreactor design and cell culture product production economics

Advanced Program: 1 Month Duration

Course Code: MM 02

Practical Learning Objectives:

  • Understand good cell culture practices followed by the industry
  • Growth of cultures – adherent and suspension cells, use of Microcarrier technology
  • Determination of  toxicity limits of different Cytotoxicity compounds  – MTT Cytotoxicity assay
  • Understand principles of cryopreservation and Nutritional requirements of cultured cells
  • Scale up of adherent cultures in 5L bioreactor
  • Current trends in Stem Cell Technology

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