We offer 3 Projects in Biotechnology. Students who have novel project ideas and would like to pursue them with us are requested to share with their project ideas by giving us a detailed outline of the project and methodology.
P1. Scale up of Pichia Pastoris using Fermentation Technology:
Duration : 1 Month
Course Code: PW 01
Pharmaceutical proteins produced via fermentation technology, i.e mass cultivation of cells in recombinant microbes provide economical system for production of therapeutic proteins such as antibiotics; vaccines blood products and etc in the biopharmaceutical sector. For this Pichia pastoris is a widely strain and is used for protein expression using recombinant DNA techniques. Scale up of Pichia pastoris In this project students analyze concepts of scale up technology and develop a protocol to scale-up from shake flask level to Lab scale fermentor and further to Pilot Scale Fermentor by optimizing the process parameters.
P2. Scale-up of Monolayer cultures using Microcarrier technology:
Duration: 1 Month
Course Code: PW 02
Monolayer cultures are adherent in nature and require a substrate for attachment while growing. Microcarrier technology is used for the scale up of adherent cultures which produce stable therapeutics. Due to large surface area, the beads are grown in suspension and are often continuously perfused with fresh medium to achieve high cell densities. In this project BHK 21 cell line which is a widely used cell line for the production of vaccines or therapeutics; are used to scale up process for mass cultivation. As the culture is of an adherent type, Microcarrier are used for scale up to Lab Scale.
P3. Purification of proteins from fermentation broth:
Duration : 1 Month
Course Code: PW 03
Protein purification is a series of critical processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Separation steps usually exploit differences in protein size, physic-chemical properties, binding affinity and biological activity. In this project the fermentation broth is subjected to primary purification techniques to remove cell debris, concentrate the broth and exchange buffer. Thereafter the secondary purification techniques are used to further purify the sample. Protein quantification techniques are carried out to identify and confirm the purified protein.
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